Microscopy

Responsible: 

Carles Justicia Mercader

Researcher

The IIBB Microscopy Unit was created in July 1999 by the supervision of Joan Serratosa, Scientific Responsible until 2020. The Unit provides services and training to researchers from the IIBB, CSIC, Clinical Campus, and other public or private research institutes on optical microscopy and image analysis. It includes a set of state-of-the-art equipment and techniques to perform the experiments requested by the different research groups.  It also provides technical assistance from the design of experiments to the analysis of the results obtained. This service is member of the Red Española de Microscopía Óptica Avanzada (REMOA).

 

STAFF/CONTACT

Scientific Responsible of Microscopy Unit: Carles Justícia.

Scientific Responsible of Confocal and Spinning Disk Microscopy: Ramón Trullàs.

Scientific Responsible of Stereological Microscopy: Roser Cortés.

Technical Responsible of Microscopy Unit: Carme Casal.

 

SERVICES OFFERED

  • Design of experiments related to optical microscopy.
  • Image acquisition on different equipment.
  • Training in the use of equipment.
  • Performing advanced microscopy techniques (live imaging…).
  • Image processing and analysis.
  • Interpretation and presentation of data obtained for scientific papers, conferences, dissemination…

 

APPLICATIONS

  • Wide-Field microscopy (bright field, dark field, phase contrast, epifluorescence).
  • Laser Scanning Confocal microscopy.
  • Spinning Disk Confocal microscopy.
  • Stereology.
  • Live imaging experiments.
  • Advanced Microscopy Techniques: photobleaching techniques [Förster Resonance Energy Transfer (FRET), Fluorescence Recovery After Photobleaching (FRAP)] and photoactivation/photoconversion techniques.
  • Image processing and analysis.

 

EQUIPMENT

  • Upright fluorescence microscope Nikon Eclipse E 1000
  • Upright light microscope Zeiss Axioplan
  • Upright fluorescence microscope Olympus BX51 for stereology
  • Inverted fluorescence microscope Olympus IX70
  • Inverted fluorescence microscope Leica DMI 4000B
  • Upright confocal microscope Leica TCS SPE
  • Inverted Andor Dragonfly Spinning disk microscope (Oxford Instruments) with motorized stage and incubation chamber.
  • Workstation equipped with ImageJ/Fiji, CellProfiler, LASAF, LASX, ZEN Blue softwares.

 

BOOKING

To book any system of our Microscopy Unit write to Technical Responsible: carme.casal@iibb.csic.es or microscopia-iibb@listas.csic.es . For the first session you have to do a technical training by microscopy staff. When you are proficient with equipment you can book them as a self-service.

All users are required to comply with Health and Safety policies and procedures of the IIBB-CSIC.

 

The Microscopy Unit of IIBB is located at 6th floor of the building. Our microscopy systems are available 12 hours a day, from 8h to 20h, 5 days a week, to all scientists working at Campus Clinic. To perform over-weekend experiments please contact our microscopy staff.

External scientists and companies have to send an official request to carme.casal@iibb.csic.es or microscopia-iibb@listas.csic.es.

 

USER DUTIES

- Read and respect the rules and instructions of microscopes usage.

- Leave the equipment and material clean and protected after use.

- Notify the technical staff about any incident or problem observed.

- Respect safety rules.

 

FEES

A 30% discount will be applied for over-night/over-weekend live imaging experiments.

Late cancellation (< 24h in advance) will result in billing if the time slot is not reserved by another user. This is no applicable to live imaging experiments.

Sala de microscopis